This standard is applicable to the determination of 7 breast milk oligosaccharides in infant formula milk powder and prepared milk powder. This standard uses high-performance liquid chromatography (HPLC). After the 7 breast milk oligosaccharides in the sample are extracted with water, different enzymatic hydrolysis conditions are used depending on whether galacto-oligosaccharides are added to the sample. After derivatization, a fluorescence detector is used. Detection, internal standard method quantification. The enzymatic hydrolysis conditions used in this method can effectively reduce the interference of galacto-oligosaccharides on sample detection; the chromatographic column used has strong chemical tolerance, and after optimization of the liquid phase conditions, it can detect the contents of infant formula milk powder and prepared milk powder. of 7 human milk oligosaccharides achieves good separation, helping ensure batch-to-batch consistency. Moreover, this method is easy to operate, has low detection cost, and can be used for routine and rapid quantitative detection.
T/SAFC 002-2024 history
2024T/SAFC 002-2024 Detection of Seven Human Milk Oligosaccharides (HMOs) in Infant Formula and Formulated Milk Powder