ASTM D5392-24
Standard Test Method for Isolation and Enumeration of Escherichia coli in Water by the Two-Step Membrane Filter Procedure

Standard No.

ASTM D5392-24

Release Date

2024

Published By

American Society for Testing and Materials (ASTM)

Lastest

ASTM D5392-24

Scope

D1193 Specification for Reagent Water D3370 Practices for Sampling Water from Flowing Process Streams D3870 Practice for Establishing Performance Characteristics for Colony Counting Methods in Microbiology (Withdrawn 2000)3 D5465 Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods 1.1 This test method describes a membrane filter (MF) procedure for the detection and enumeration of Escherichia coli, a bacterium found exclusively in the feces of humans and other warm-blooded animals. The presence of these microorganisms in water is an indication of fecal pollution and the possible presence of enteric pathogens. These bacteria are found in water and wastewater in a wide range of densities. The detection limit of this procedure is one colony forming unit (CFU) per volume filtered. 3. Terminology 3.1 Definitions: 3.1.1 For definitions of terms used in this standard, refer to Terminology D1129. 1.2 This test method has been used successfully with temperate fresh and marine ambient waters, and wastewaters. It is the user’s responsibility to ensure the validity of this test method for waters of other types. 3.2 Definitions of Terms Specific to This Standard: 3.2.1 Escherichia coli (E. coli), n—a species of bacteria that is a member of the total coliform group and known to originate in the feces of warm-blooded animals. 1.3 The values stated in SI units are to be regarded as standard. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For specific hazard statements, see Section 9. 1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. 3.3 Performance Characteristics (Practice D3870): 3.3.1 accuracy, n—the proportion of the observed count to the true density of a sample. 3.3.2 bias, n—the persistent positive or negative deviation of the average value of the test method from the assumed or accepted true value. 3.3.3 precision, n—the degree of agreement of repeated measurements of the same parameter expressed quantitatively as the standard deviation or as the 95 % confidence limits of the mean computed from the results of a series of controlled determinations.

ASTM D5392-24 Referenced Document

• ASTM D1129 Standard Terminology Relating to Water

ASTM D5392-24 history

• 2024 ASTM D5392-24 Standard Test Method for Isolation and Enumeration of Escherichia coli in Water by the Two-Step Membrane Filter Procedure
• 2019 ASTM D5392-19 Standard Test Method for Isolation and Enumeration of Escherichia coli in Water by the Two-Step Membrane Filter Procedure
• 2014 ASTM D5392-14 Standard Test Method for Isolation and Enumeration of Escherichia Coli in Water by the Two-Step Membrane Filter Procedure
• 1993 ASTM D5392-93(2006) Standard Test Method for Isolation and Enumeration ofEscherichia Coli in Water by the Two-Step Membrane Filter Procedure
• 2000 ASTM D5392-93(2000) Standard Test Method for Isolation and Enumeration of Escherichia Coli in Water by the Two-Step Membrane Filter Procedure