BS PD ISO/TS 21569-3:2020
Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products-Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening for genetically m...

Scope: This document describes a procedure for the detection of the DNA transition sequence between the 35S promotor (P35S) from Cauliflower mosaic virus and a modified phoshinothricin-acetyltransferase gene (pat) from Streptomyces viridochromogenes. The P35S-pat construct is frequently found in genetically modified plants with tolerance for phosphinothricin-containing herbicides. The P35S-pat construct specific method is based on a real-time PCR and can be used for qualitative and quantitative screening purposes. For identification and quantification of a specific event, a follow-up analysis can be carried out.

BS PD ISO/TS 21569-3:2020 history

2020 BS PD ISO/TS 21569-3:2020 Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products-Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening for genetically m...
2015 BS PD ISO/TS 21569-3:2015 Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products. Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening genetically modifie