1Key Takeaways
The standard specifies the key steps of a quantitative polymerase chain reaction (qPCR) method for measuring the abundance of selected microbial gene sequences extracted from soil DNA, providing an estimate of the targeted microbial groups. It is noteworthy that gene copy number is not necessarily correlated with the n…
2Scope / Description
The standard specifies the key steps of a quantitative polymerase chain reaction (qPCR) method for measuring the abundance of selected microbial gene sequences extracted from soil DNA, providing an estimate of the targeted microbial groups. It is noteworthy that gene copy number is not necessarily correlated with the number of organisms measured. For instance, the number of ribosomal operon copies can vary from 1 to 20 among different bacterial strains. Consequently, the quantified number of 16S rRNA gene sequences in extracted soil DNA does not yield an exact estimate of the soil bacterial count. Furthermore, gene sequence abundance is not necessarily indicative of viable microorganisms and may also include sequences amplified from dead microbial cells.
6Frequently Asked Questions
What is UNI EN ISO 17601:2026?
UNI EN ISO 17601:2026 — Soil quality - Estimation of abundance of selected microbial gene sequences by quantitative polymerase chain reaction (qPCR) from DNA directly extracted from soil is an international standard developed by Ente Nazionale Italiano di Unificazione. The standard specifies the key steps of a quantitative polymerase chain reaction (qPCR) method for measuring the abundance of selected microbial gene sequences extracted from soil DNA, providing an estimate of the targeted microbial groups. It is...
What does UNI EN ISO 17601:2026 cover?
This standard covers: The standard specifies the key steps of a quantitative polymerase chain reaction (qPCR) method for measuring the abundance of selected microbial gene sequences extracted from soil DNA, providing an estimate of the targeted microbial groups. It is noteworthy that gene copy number is not necessarily...
Who should use this standard?
This standard is intended for organizations, professionals, and stakeholders involved in various industries and sectors. It is applicable to manufacturers, service providers, regulatory bodies, and certification organizations.
What is the latest version of UNI EN ISO 17601:2026?
The current published version is UNI EN ISO 17601:2026, published on 2026-06-11 00:00:00. Always check for amendments or pending revisions.
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