Soil quality — Sampling of soil invertebrates — Part 1: Hand-sorting and extraction of earthworms

This document establishes a standardized procedure for sampling soil invertebrates, specifically focusing on earthworms. It outlines the manual techniques required to extract and separate earthworms from soil samples within their natural habitat. The methodology is designed to ensure consistency and reproducibility in field operations, facilitating accurate assessments of soil biodiversity. The approach includes detailed steps for soil extraction, sieving, and manual sorting processes. It aims to support scientific research and environmental monitoring efforts by providing a uniform framework for data collection. This protocol addresses key aspects of handling soil samples to minimize disturbance and maximize recovery rates. By adhering to these guidelines, practitioners can generate reliable datasets regarding earthworm populations. The procedures are applicable across various soil types and environmental conditions, contributing to global efforts in understanding soil health and ecosystem dynamics.