Food Products. Determination of fumonisin B1 and fumonisin B2 in corn-based foods for infants and young children. HPLC method with immunoaffinity column purification and fluorescence detection after precolumn derivatization

This document establishes a high-performance liquid chromatography method for determining ochratoxin A and ochratoxin B in infant foods based on processed corn. The procedure incorporates immunosorbent column purification followed by derivatization and fluorescence detection to ensure accurate quantification of these mycotoxins. Designed for applications in food safety monitoring, the method provides a structured framework for laboratory analysis within the scope of infant nutrition products. It outlines specific requirements for sample preparation, reagent selection, instrument calibration, and data interpretation to maintain consistency across testing facilities. The approach aims to enhance the reliability of检测结果 for contaminants that pose potential health risks to young children. By defining clear operational parameters, the protocol supports the verification of compliance with safety thresholds in commercial and regulatory contexts. The method addresses the challenges associated with low concentration levels in complex food matrices through the use of specialized purification techniques. Implementation of this approach facilitates the exchange of analytical results and promotes harmonized testing practices among member organizations engaged in food quality assurance.