Caseins and caseinates; Determination of lactose content; Photometric method

This document outlines a methodology for determining the lactose content in casein and caseinates using a photometric technique. The procedure is designed to provide a reliable analytical approach for assessing the composition of dairy protein derivatives. By utilizing light absorption measurements, the method enables precise quantification of residual sugars within these processed protein products. The approach involves specific sample preparation steps to ensure accurate results, followed by a measurement phase where the intensity of light passing through the sample solution is analyzed. This analytical technique serves as a standardized means for quality control and compliance verification in the food industry. It establishes the necessary protocols for reagents, equipment calibration, and calculation procedures to ensure consistency across different testing environments. The methodology focuses on the chemical interactions that allow for the detection and measurement of lactose levels without interference from other components present in the protein matrix. Adherence to these guidelines facilitates uniformity in analytical reporting and supports the evaluation of product purity.